A study on total RNA including microRNA isolation from cell lines by different cost effective methods

Isolation of microarray-grade total RNA, microRNA, and DNA from a single PAXgene blood RNA tube.

We have developed a procedure for isolation of microRNA and genomic DNA in addition to total RNA from whole blood stabilized in PAXgene Blood RNA tubes. The procedure is based on automatic extraction on a BioRobot MDx and includes isolation of DNA from a fraction of the stabilized blood and recovery of small RNA species that are otherwise lost. The procedure presented here is suitable for large...

RNA isolation (Total RNA Extraction from High Polysaccharide Plants). Fatemeh Mehrpooyan*

Simple method of RNA isolation from human leucocytic cell lines.

Very reliable methods have been developed to extract RNA from different tissues and cell lines (1, 2, 3). RNA preparation, however, is still cumbersome and time consuming, especially when extracting a large number of cells (>10) and cell samples. The isolation of RNA from leucocytic cells is particularly difficult for three reasons: a) high levels of endogenous RNase; b) low amount of RNA (RNA/...

reflections on taught courses of the iranian ma program in english translation: a mixed-methods study

the issue of curriculum and syllabus evaluation and revision has been in center of attention right from when curriculum came into attention of educational institutions. thus everywhere in the world in educational institutions curricula and syllabi are evaluated and revised based on the goals, the needs, existing content, etc.. in iran any curriculum is designed in a committee of specialists and...

Drop-on-Demand Single Cell Isolation and Total RNA Analysis

Technologies that rapidly isolate viable single cells from heterogeneous solutions have significantly contributed to the field of medical genomics. Challenges remain both to enable efficient extraction, isolation and patterning of single cells from heterogeneous solutions as well as to keep them alive during the process due to a limited degree of control over single cell manipulation. Here, we ...